Variation in complement fixation test results with three Histoplasma capsulatum yeast phase antigens
نویسندگان
چکیده
منابع مشابه
Variation in complement fixation test results with three Histoplasma capsulatum yeast phase antigens.
Antigens derived from three different yeast strains of Histoplasma capsulatum (GW and VC used by the Veterans Administration Serology Reference Laboratory and the Centers for Disease Control reference antigen, A811) were compared with each other to determine their equivalency. Results from 200 sera, tested concurrently by the Serology Reference Laboratory and the University of Kentucky Mycosero...
متن کاملEvaluation of histoplasmin and yeast phase antigens derived from a single strain of Histoplasma capsulatum in the complement fixation test.
A complement fixation procedure for the serological diagnos of histoplasmosis in a human patient was attempted first in 1941 by Van Pernis, Benson, and Hollinger. The results were negative with an antigen which consisted of the undiluted filtrate (histoplasmin) from a glucose broth medium inoculated with Hidtoplasma capsulatum. Since that time other types of antigens have been developed by vari...
متن کاملRibonucleic acid polymerases of the yeast phase of Histoplasma capsulatum.
Ribonucleic acid (RNA) polymerases of Histoplasma capsulatum (yeast phase) were fractionated by phosphocellulose chromatography and partially characterized. Three distinct, active fractions were seen. The major RNA polymerase species was inhibited strongly by alpha-amanitin, whereas the other two were resistant. When either slightly purified (HSE) extract or the major active component was assay...
متن کاملRespiration in the yeast and mycelial phases of Histoplasma capsulatum.
Respiration in the yeast and mycelial phases of Histoplasma capsulatum proceeds via a cytochrome system and an alternate oxidase, both present constitutively. The mycelial cytochrome system is distinguished by an additional partial shunt around the antimycin-sensitive site.
متن کاملProduction and characterization of murine monoclonal antibodies to Histoplasma capsulatum yeast cell antigens.
Four monoclonal antibodies (MAbs) were produced by immunizing mice with a disrupted yeast cell homogenate of Histoplasma capsulatum. MAbs 1 and 2 reacted only with the yeast cell antigens of H. capsulatum and Blastomyces dermatitidis, whereas MAbs 3 and 4 showed broader cross-reactivity. MAb 3 cross-reacted with B. dermatitidis, Paracoccidioides brasiliensis, Sporothrix schenckii, and Candida a...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1985
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.22.6.1066-1067.1985